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avidin peroxidase  (Vector Laboratories)


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    Vector Laboratories avidin peroxidase
    Avidin Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+peroxidase/Avidin+D%2C+Horseradish+Peroxidase+(Av-HRP)%2C+Concentrate%2C+for+IHC/bio_rxiv__64898__2026__04__23__720463-60-13-15
    Average 93 stars, based on 295 article reviews
    avidin peroxidase - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: An injectable and 3D printable pro-chondrogenic hyaluronic acid and collagen type II composite hydrogel for the repair of articular cartilage defects.
    Article Snippet: The sections were then rinsed with PBS and incubated with a mouse primary antibody for either COL1 (ab6308, Abcam, UK) or COL2 (SC52658, Santa Cruz, USA) at a 1:200 or 1:100 dilution respectively in IHC blocking buffer at 4 ◦C overnight. .. The following day, the sections were rinsed with PBS and incubated with a goat anti-mouse IgG antibody conjugated with horseradish peroxidase (HRP) (ab6728, Abcam, UK) at a 1:500 dilution in IHC blocking buffer at room temperature for 1 h. The sections were then rinsed with PBS and treated with avidin peroxidase as per the manufacturer’s protocol (PK-6101, Vector Laboratories, USA) and rinsed with PBS again before being treated with a 3,3′-Diaminobenzidine (DAB) substrate peroxidase kit (SK-4100, Vector Laboratories, USA) for 10 min. .. Finally, the sections were rinsed with PBS, dehydrated in 100% (v/v) ethanol and xylene and mounted using DPX (Sigma Aldrich, Ireland) and coverslips.

    Article Title: NKT Cells Stimulated by Long Fatty Acyl Chain Sulfatides Significantly Reduces the Incidence of Type 1 Diabetes in Nonobese Diabetic Mice
    Article Snippet: .. The wells were then incubated with biotin-conjugated anti-IFN-g or anti-IL-4 mAbs followed by incubation with avidin peroxidase (Vector Laboratories). .. Spots were developed by the addition of 3-amino-9-ethylcarbazole substrate (Sigma-Aldrich), and were counted using a computerized image analysis system and the image analyzer program, NIH Image 1.61.

    Article Title: Molecular Iodine Induces Anti- and Pro-Neoplastic Effects in Prostate Cancer Models
    Article Snippet: .. After 3 × T-PBS rinses, slides were incubated with their corresponding secondary antibodies for 2 h. Then, avidin peroxidase was coupled to biotinylated secondary antibodies for 1 h incubation in the dark with ABC reagents (Vectastains PK-6101 & PK-6102; Vector laboratories, Newark, CA, USA), followed by 3 × PBS rinses and incubation with DAB substrate (Vector laboratories SK-4100). ..

    Article Title: Cerebral Changes Occurring in Arginase and Dimethylarginine Dimethylaminohydrolase (DDAH) in a Rat Model of Sleeping Sickness
    Article Snippet: After transfer, membranes were first saturated for one hour at room temperature in buffer A (Tris 50 mM pH 7.5, NaCl 166 mM, Tween 0.2%) containing 5% of non fat dry milk, followed by an incubation with the rabbit polyclonal anti-DDAH-1 (1/500, Orbigen, San Diego, USA), rabbit polyclonal anti-DDAH-2 (H-85 1/200, Santa Cruz Biotechnology, California, USA), rabbit polyclonal anti-arginase-1 (H-52, 1/200, Santa Cruz Biotechnology), rabbit polyclonal anti-arginase-2 (H-64, 1/200, Santa Cruz Biotechnology) or mouse anti-β actin antibody (1/1000, Santa Cruz Biotechnology) for 6 hours in buffer A containing 2.5% of non fat dry milk. .. After three washings in buffer A, membranes were incubated during one hour with corresponding biotinylated secondary antibody, goat anti-rabbit (1/2000, Vector Labs, ABCYS, Paris, France), or goat-anti mouse (1/1000, Vector Labs) in buffer A containing 2.5% non fat dry milk, washed again in buffer A, then incubated for two hours with avidin-peroxidase (1/1000, Vectastain kit, Vector Labs) in buffer A. Blots were then washed three times and the conjugates were visualized by enhanced chemiluminescence (ECL) (Super Signal West Pico, Pierce, Thermo Scientific, and Courtabeuf, France). .. The ECL signals were acquired with ChemidocXRS (BioRad, Marnes-la-Coquette, France) and quantified with Quantity One software (BioRad).

    Article Title: Delineating the effects of prenatal oxycodone exposure and melatonin treatment on placental and fetal outcomes in pregnant rats
    Article Snippet: Following overnight incubation, sections were washed in 1X TBST (0.6% Tween-20) and incubated for one hour with appropriate secondary antibodies (goat anti-mouse; Vector Lab #BA-9200 or goat anti-rabbit; Vector Lab #BA-1000). .. After three additional washes with 1X TBST (0.6% Tween-20), sections were incubated with avidin-peroxidase (1:200; Vector Laboratories #A-2004) for 30 minutes. .. Signal was developed using 3,3’-diaminobenzidine (Sigma-Aldrich #D4293) for 1 to 2 minutes, followed by rinsing in water, counterstaining with hematoxylin (Stat Lab #95-1), dehydration, and mounted using CoverSafe mounting medium (Stat Lab #MMC0226).

    Immunohistochemistry:

    Article Title: An injectable and 3D printable pro-chondrogenic hyaluronic acid and collagen type II composite hydrogel for the repair of articular cartilage defects.
    Article Snippet: The sections were then rinsed with PBS and incubated with a mouse primary antibody for either COL1 (ab6308, Abcam, UK) or COL2 (SC52658, Santa Cruz, USA) at a 1:200 or 1:100 dilution respectively in IHC blocking buffer at 4 ◦C overnight. .. The following day, the sections were rinsed with PBS and incubated with a goat anti-mouse IgG antibody conjugated with horseradish peroxidase (HRP) (ab6728, Abcam, UK) at a 1:500 dilution in IHC blocking buffer at room temperature for 1 h. The sections were then rinsed with PBS and treated with avidin peroxidase as per the manufacturer’s protocol (PK-6101, Vector Laboratories, USA) and rinsed with PBS again before being treated with a 3,3′-Diaminobenzidine (DAB) substrate peroxidase kit (SK-4100, Vector Laboratories, USA) for 10 min. .. Finally, the sections were rinsed with PBS, dehydrated in 100% (v/v) ethanol and xylene and mounted using DPX (Sigma Aldrich, Ireland) and coverslips.

    Blocking Assay:

    Article Title: An injectable and 3D printable pro-chondrogenic hyaluronic acid and collagen type II composite hydrogel for the repair of articular cartilage defects.
    Article Snippet: The sections were then rinsed with PBS and incubated with a mouse primary antibody for either COL1 (ab6308, Abcam, UK) or COL2 (SC52658, Santa Cruz, USA) at a 1:200 or 1:100 dilution respectively in IHC blocking buffer at 4 ◦C overnight. .. The following day, the sections were rinsed with PBS and incubated with a goat anti-mouse IgG antibody conjugated with horseradish peroxidase (HRP) (ab6728, Abcam, UK) at a 1:500 dilution in IHC blocking buffer at room temperature for 1 h. The sections were then rinsed with PBS and treated with avidin peroxidase as per the manufacturer’s protocol (PK-6101, Vector Laboratories, USA) and rinsed with PBS again before being treated with a 3,3′-Diaminobenzidine (DAB) substrate peroxidase kit (SK-4100, Vector Laboratories, USA) for 10 min. .. Finally, the sections were rinsed with PBS, dehydrated in 100% (v/v) ethanol and xylene and mounted using DPX (Sigma Aldrich, Ireland) and coverslips.

    Article Title: Development of a 3D-printed bioabsorbable composite scaffold with mechanical properties suitable for treating large, load-bearingarticular cartilage defects
    Article Snippet: Sections were rinsed with PBS before incubation with goat anti-mouse IgG antibodies conjugated with HRP (ab6728, Abcam) in a 1:500 dilution for 1 h at room temperature. .. Samples were treated with avidin peroxidase, following the manufacturer's protocol (PK-6101, Vector Laboratories, USA), in blocking buffer (45 min) and rinsed with PBS. .. Then, they were treated with a DAB substrate peroxidase kit (SK-4100, Vector Laboratories, USA) for 12 min. Next, sections were rinsed with PBS, 100 % ethanol and xylene, before being mounted using DPX (Sigma-Aldrich) and coverslips.

    Avidin-Biotin Assay:

    Article Title: An injectable and 3D printable pro-chondrogenic hyaluronic acid and collagen type II composite hydrogel for the repair of articular cartilage defects.
    Article Snippet: The sections were then rinsed with PBS and incubated with a mouse primary antibody for either COL1 (ab6308, Abcam, UK) or COL2 (SC52658, Santa Cruz, USA) at a 1:200 or 1:100 dilution respectively in IHC blocking buffer at 4 ◦C overnight. .. The following day, the sections were rinsed with PBS and incubated with a goat anti-mouse IgG antibody conjugated with horseradish peroxidase (HRP) (ab6728, Abcam, UK) at a 1:500 dilution in IHC blocking buffer at room temperature for 1 h. The sections were then rinsed with PBS and treated with avidin peroxidase as per the manufacturer’s protocol (PK-6101, Vector Laboratories, USA) and rinsed with PBS again before being treated with a 3,3′-Diaminobenzidine (DAB) substrate peroxidase kit (SK-4100, Vector Laboratories, USA) for 10 min. .. Finally, the sections were rinsed with PBS, dehydrated in 100% (v/v) ethanol and xylene and mounted using DPX (Sigma Aldrich, Ireland) and coverslips.

    Article Title: NKT Cells Stimulated by Long Fatty Acyl Chain Sulfatides Significantly Reduces the Incidence of Type 1 Diabetes in Nonobese Diabetic Mice
    Article Snippet: .. The wells were then incubated with biotin-conjugated anti-IFN-g or anti-IL-4 mAbs followed by incubation with avidin peroxidase (Vector Laboratories). .. Spots were developed by the addition of 3-amino-9-ethylcarbazole substrate (Sigma-Aldrich), and were counted using a computerized image analysis system and the image analyzer program, NIH Image 1.61.

    Article Title: Molecular Iodine Induces Anti- and Pro-Neoplastic Effects in Prostate Cancer Models
    Article Snippet: .. After 3 × T-PBS rinses, slides were incubated with their corresponding secondary antibodies for 2 h. Then, avidin peroxidase was coupled to biotinylated secondary antibodies for 1 h incubation in the dark with ABC reagents (Vectastains PK-6101 & PK-6102; Vector laboratories, Newark, CA, USA), followed by 3 × PBS rinses and incubation with DAB substrate (Vector laboratories SK-4100). ..

    Article Title: Prenatal Cocaine Exposure Increases Synaptic Localization of a Neuronal RasGEF, GRASP-1 via Hyperphosphorylation of AMPAR Anchoring Protein, GRIP
    Article Snippet: Target Buffer was purchased from Dako (Carpenturia, CA). .. Avidin-peroxidase-labeled biotin complex (ABC) was from Vector Labs (Foster City, CA). .. 3-3-diaminobenzidine-4 HCl (DAB)/H 2 O 2 was from Biomeda (Foster City, CA).

    Article Title: Cerebral Changes Occurring in Arginase and Dimethylarginine Dimethylaminohydrolase (DDAH) in a Rat Model of Sleeping Sickness
    Article Snippet: After transfer, membranes were first saturated for one hour at room temperature in buffer A (Tris 50 mM pH 7.5, NaCl 166 mM, Tween 0.2%) containing 5% of non fat dry milk, followed by an incubation with the rabbit polyclonal anti-DDAH-1 (1/500, Orbigen, San Diego, USA), rabbit polyclonal anti-DDAH-2 (H-85 1/200, Santa Cruz Biotechnology, California, USA), rabbit polyclonal anti-arginase-1 (H-52, 1/200, Santa Cruz Biotechnology), rabbit polyclonal anti-arginase-2 (H-64, 1/200, Santa Cruz Biotechnology) or mouse anti-β actin antibody (1/1000, Santa Cruz Biotechnology) for 6 hours in buffer A containing 2.5% of non fat dry milk. .. After three washings in buffer A, membranes were incubated during one hour with corresponding biotinylated secondary antibody, goat anti-rabbit (1/2000, Vector Labs, ABCYS, Paris, France), or goat-anti mouse (1/1000, Vector Labs) in buffer A containing 2.5% non fat dry milk, washed again in buffer A, then incubated for two hours with avidin-peroxidase (1/1000, Vectastain kit, Vector Labs) in buffer A. Blots were then washed three times and the conjugates were visualized by enhanced chemiluminescence (ECL) (Super Signal West Pico, Pierce, Thermo Scientific, and Courtabeuf, France). .. The ECL signals were acquired with ChemidocXRS (BioRad, Marnes-la-Coquette, France) and quantified with Quantity One software (BioRad).

    Article Title: Delineating the effects of prenatal oxycodone exposure and melatonin treatment on placental and fetal outcomes in pregnant rats
    Article Snippet: Following overnight incubation, sections were washed in 1X TBST (0.6% Tween-20) and incubated for one hour with appropriate secondary antibodies (goat anti-mouse; Vector Lab #BA-9200 or goat anti-rabbit; Vector Lab #BA-1000). .. After three additional washes with 1X TBST (0.6% Tween-20), sections were incubated with avidin-peroxidase (1:200; Vector Laboratories #A-2004) for 30 minutes. .. Signal was developed using 3,3’-diaminobenzidine (Sigma-Aldrich #D4293) for 1 to 2 minutes, followed by rinsing in water, counterstaining with hematoxylin (Stat Lab #95-1), dehydration, and mounted using CoverSafe mounting medium (Stat Lab #MMC0226).

    Article Title: Development of a 3D-printed bioabsorbable composite scaffold with mechanical properties suitable for treating large, load-bearingarticular cartilage defects
    Article Snippet: Sections were rinsed with PBS before incubation with goat anti-mouse IgG antibodies conjugated with HRP (ab6728, Abcam) in a 1:500 dilution for 1 h at room temperature. .. Samples were treated with avidin peroxidase, following the manufacturer's protocol (PK-6101, Vector Laboratories, USA), in blocking buffer (45 min) and rinsed with PBS. .. Then, they were treated with a DAB substrate peroxidase kit (SK-4100, Vector Laboratories, USA) for 12 min. Next, sections were rinsed with PBS, 100 % ethanol and xylene, before being mounted using DPX (Sigma-Aldrich) and coverslips.

    Article Title: Adult telomerase positive stem cells: Effects of biological agents and characterization criteria on genomically-labeled clones and unlabeled clones of adult cells
    Article Snippet: • Biotin, goat anti-mouse IgG, #OB1126-21, Fisher • Biotin, goat anti-mouse IgG (fab specific), #B-7151, Sigma • Biotin, goat anti-rabbit IgG, #OB1316-21, Sigma • Biotin-SP, goat anti-mouse IgG, #JGM-065003, Accurate • Biotin-SP, goat anti-mouse fab-specific, #JGM-066003, Accurate • Biotinylated affinity purified, rat adsorbed anti-mouse IgG (H + L) (BA-2001, Vector Laboratories) • Goat anti-rat osteocalcin, #BT-413, Biomedical Technology .. • Avidin-peroxidase, #JSA-030083, Accurate • Horseradish peroxidase conjugated Avidin-D, #A-2004, Vector • Monoclonal anti-goat IgG clone GT-34 biotin, #B-3148, Sigma • Peroxidase Standard PK-4000 Vastatin ABC Reagent Kit, Vector Laboratories • Streptavidin-peroxidase, #JSA-030084, Accurate .. • ABTS kit, #506201, Kirkegaard and Perry • 4-Cloro-1-napthol, #C-8890, Sigma • 3,5-Diaminobenzoic acid dihydrochloride, #11-383-2, Aldrich • Vastatin ABC Reagent Kit, Vector Laboratories Inc., Burlingame, CA



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